Capacidad para crecer en alcoholes y compuestos aromáticos de bacterias
metilotroficas aisladas del humedal.
La Mixtequilla, Veracruz
Antonia Correa Basurto;
MarÃa Teresa Núñez-Cardona;
Jaime GarcÃa Mena;
Alfonso Esquivel Herrera Palabras clave (keywords):
bacterias metilotroficas,
humedal, metanol,
alcoholes,
compuestos aromáticos,
methylotropic bacteria,
wetland,
methanol,
alcohols,
aromatic compounds
Sociedades Rurales, Producción y Medio Ambiente
Vol. 12 No. 23; 2012
Págs. 161-178
Resumen
Resumen. Se evaluó la capacidad de bacterias metilotroficas acuáticas del humedal La Mixtequilla (Veracruz), para crecer en alcoholes y compuestos aromáticos.
Para aislarlas se utilizó un medio con metanol y se observó su crecimiento a concentraciones diferentes de metanol, etanol, 1-butanol, 2-propanol, tolueno y xileno (0.5-4 mL/L). Los cultivos fueron caracterizados e identificados genéticamente.
Se obtuvieron 38 cultivos de bacterias metilotroficas que crecieron en todas las concentraciones de alcoholes y compuestos aromáticos ensayados.
Nueve aislados fueron identificados genéticamente y sólo cinco de éstos fueron caracterizados catabólicamente, el análisis de su 16S rDNA reveló que fueron similares a Tetrasphaera sp., Bacillus pumilus, B. jeotgali, Enterobacter asburiae Klebsiella pneumoniae y Serratia marcescens. Estos cultivos podrÃan ser útiles para biorremediar ambientes impactados negativamente por
actividades humanas.
Abstract
Abstract. The growth capability on alcohols and aromatic compounds was assessed
for bacteria isolated from water samples of the wetland La Mixtequilla
(Veracruz). Methylotrophic activity of bacterial isolates was detected through
their ability to grow on a culture medium with methanol as sole carbon and
energy source. Isolates were purified before testing their ability to grow on methanol,
ethanol, 1-butanol, 2-propanol, toluene and xylene as sole carbon sources.
These isolates were catabolically characterized and genetically identified;
bacterial growth was detected in all the alcohols and aromatic compounds, at
all concentrations which they were assayed; nine of the isolates were genetically
identified and five of them were catabolically characterized. The 16S rDNA
analysis revealed that they were similar to Tetrasphaera sp., Bacillus pumilus,
B. jeotgali, Enterobacter asburiae Klebsiella pneumoniae y Serratia marcescens.
These isolates could be useful for bioremediation of environments negatively impacted
by human activities
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